View source for DNA repair protein RAD51 homolog 1 (Mus musculus)

{| align="left"
  | __TOC__
  |}
{{#invoke:InfoboxforTarget|run|RAD51|[https://www.uniprot.org/uniprot/Q08297 Q08297]|Mus musculus|Cys319|RecA family,<br/>[http://pfam.xfam.org/family/PF08423 RAD51 subfamily]|[[:Category:DNA repair protein RAD51 homolog 1 (Mus musculus)|Ligand list]]|DNA repair enzyme}}
==Summary==

===Protein Function ===
RAD51 plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PMID: 28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PMID: 26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PMID: 26253028). (From Uniprot)
<br/>

===Cys Function & Property===
From the homologous structures, Cys319 is very close to the ATP and metal ion binding site in space.<br/>

* Hydrophobic property:
:[[File:513-hydro.png||600px]]
* SASA：
:Cys319: Unknown<br/>

==Protein Sequence==
<font face="Courier">
MAMQMQLEAS ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGYHTVE <br/>
AVAYAPKKEL INIKGISEAK ADKILTEAAK LVPMGFTTAT EFHQRRSEII <br/>
QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR <br/>
GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARGFNTDHQ <br/>
TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR <br/>
MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL <br/>
YLRKGRGETR ICKIYDSP<span style="background:#ffff00">'''C'''</span>L PEAEAMFAIN ADGVGDAKD    <br/> 
</font>

==Structural Information==
*Known structure with covalent ligand: <br/>
:Unknown<br/>

*Protein structure:
:Unknown<br/>

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko03440 Homologous recombination] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko03460 Fanconi anemia pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05200 Pathways in cancer] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05212 Pancreatic cancer] <br/>

==Experimental Evidence==
:Fluorescence Polarization Assay, Cys-directed Mutation

==Reference==
# Budke B, Logan H L, Kalin J H, et al. '''RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells[J].''' Nucleic acids research, 2012, 40(15): 7347-7357. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22573178 22573178]<br/>

[[Category:Targets]]
[[Category:Mus musculus]]
[[Category:DNA repair enzyme]]
[[Category:RAD51 subfamily]]
[[Category:RecA family]]
[[Category:Homologous recombination]]
[[Category:Fanconi anemia pathway]]
[[Category:Pathways in cancer]]
[[Category:Pancreatic cancer]]