View source for Glyceraldehyde-3-phosphate dehydrogenase (Mus musculus)

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{{#invoke:InfoboxforTarget|run|Gapdh|[https://www.uniprot.org/uniprot/P16858 P16858]|Mus musculus|Cys154|Glyceraldehyde-3-phosphate dehydrogenase family|[[:Category:Glyceraldehyde-3-phosphate dehydrogenase (Mus musculus)|Ligand list]]|Metabolic enzyme}}

==Summary==

===Protein Function ===
Under normal cellular conditions, cytoplasmic GAPDH exists primarily as a tetramer. This form is composed of four identical 37-kDa subunits containing a single catalytic thiol group each and critical to the enzyme's catalytic function. Nuclear GAPDH has increased isoelectric point (pI) of pH 8.3–8.7. Of note, the cysteine residue Cys152 in human GAPDH's (Cys154 in mouse) active site is required for the induction of apoptosis by oxidative stress. Notably, post-translational modifications of cytoplasmic GAPDH contribute to its functions outside of glycolysis. (From Wikipedia) <br/>
[[File:566-function.png|center|400px]]
<div align="center">The reaction of GAPDH catalyzed</div>

===Cys Function & Property===
Cys154 is the active site and very close to one of the Glyceraldehyde 3-phosphate binding sites of GAPDH, residues 149-151. <br/>

* Hydrophobic property:
:[[File:566-hydro.png||600px]]
* SASA：
:Cys154: Unknown <br/>

==Protein Sequence==
<font face="Courier">
MVKVGVNGFG RIGRLVTRAA ICSGKVEIVA INDPFIDLNY MVYMFQYDST <br/>
HGKFNGTVKA ENGKLVINGK PITIFQERDP TNIKWGEAGA EYVVESTGVF <br/>
TTMEKAGAHL KGGAKRVIIS APSADAPMFV MGVNHEKYDN SLKIVSNASC <br/>
TTN<span style="background:#ffff00">'''C'''</span>LAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA <br/>
AQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTPNVSV VDLTCRLEKP <br/>
AKYDDIKKVV KQASEGPLKG ILGYTEDQVV SCDFNSNSHS STFDAGAGIA <br/>
LNDNFVKLIS WYDNEYGYSN RVVDLMAYMA SKE                   <br/> 
</font>

==Structural Information==
*Known structure with covalent ligand: <br/>
:Unknown<br/>  

*Protein structure:
:Unknown<br/>  

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko00010 Glycolysis/Gluconeogenesis] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko01100 Metabolic pathways] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko01200 Carbon metabolism] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko01230 Biosynthesis of amino acids] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04066 HIF-1 signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05010 Alzheimer disease] <br/>

==Experimental Evidence==
:UPLC-MS, Two-dimensional SDS–PAGE 

==Reference==
# Miura T, Shinkai Y, Hirose R, et al. '''Glyceraldehyde-3-phosphate dehydrogenase as a quinone reductase in the suppression of 1, 2-naphthoquinone protein adduct formation[J].''' Free Radical Biology and Medicine, 2011, 51(11): 2082-2089. [https://www.ncbi.nlm.nih.gov/pubmed/?term=21963991 21963991]<br/>

[[Category:Targets]]
[[Category:Mus musculus]]
[[Category:Metabolic enzyme]]
[[Category:Glyceraldehyde-3-phosphate dehydrogenase family]]
[[Category:Glycolysis/Gluconeogenesis]]
[[Category:Metabolic pathways]]
[[Category:Carbon metabolism]]
[[Category:Biosynthesis of amino acids]]
[[Category:HIF-1 signaling pathway]]
[[Category:Alzheimer disease]]