View source for Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

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{{#invoke:InfoboxforTarget|run|ATP2A1, SERCA1, Calcium pump 1|[https://www.uniprot.org/uniprot/O14983 O14983]|Homo sapiens|Cys674|Cation transport ATPase (P-type) family, Type IIA subfamily|[[:Category:Sarcoplasmic/endoplasmic reticulum calcium ATPase 1|Ligand list]]|Ion channel}}
==Summary==

===Protein Function ===
Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (From Uniprot).<br/>
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. <br/>
SERCA Ca(2+)-ATPase is a intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. (From Wikipedia)
<br/>

===Cys Function & Property===
Cys674 is very close to the ATP binding site in sequence.<br/>

* Hydrophobic property:
:[[File:523-hydro.png||600px]]
* SASA：
:Cys674: Unknown

==Protein Sequence==
<font face="Courier">
MEAAHAKTTE ECLAYFGVSE TTGLTPDQVK RNLEKYGLNE LPAEEGKTLW <br/>
ELVIEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILIA <br/>
NAIVGVWQER NAENAIEALK EYEPEMGKVY RADRKSVQRI KARDIVPGDI <br/>
VEVAVGDKVP ADIRILAIKS TTLRVDQSIL TGESVSVIKH TEPVPDPRAV <br/>
NQDKKNMLFS GTNIAAGKAL GIVATTGVGT EIGKIRDQMA ATEQDKTPLQ <br/>
QKLDEFGEQL SKVISLICVA VWLINIGHFN DPVHGGSWFR GAIYYFKIAV <br/>
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS <br/>
DKTGTLTTNQ MSVCKMFIID KVDGDICLLN EFSITGSTYA PEGEVLKNDK <br/>
PVRPGQYDGL VELATICALC NDSSLDFNEA KGVYEKVGEA TETALTTLVE <br/>
KMNVFNTDVR SLSKVERANA CNSVIRQLMK KEFTLEFSRD RKSMSVYCSP <br/>
AKSSRAAVGN KMFVKGAPEG VIDRCNYVRV GTTRVPLTGP VKEKIMAVIK <br/>
EWGTGRDTLR CLALATRDTP PKREEMVLDD SARFLEYETD LTFVGVVGML <br/>
DPPRKEVTGS IQLCRDAGIR VIMITGDNKG TAIAICRRIG IFGENEEVAD <br/>
RAYTGREFDD LPLAEQREAC RRA<span style="background:#ffff00">'''C'''</span>CFARVE PSHKSKIVEY LQSYDEITAM <br/>
TGDGVNDAPA LKKAEIGIAM GSGTAVAKTA SEMVLADDNF STIVAAVEEG <br/>
RAIYNNMKQF IRYLISSNVG EVVCIFLTAA LGLPEALIPV QLLWVNLVTD <br/>
GLPATALGFN PPDLDIMDRP PRSPKEPLIS GWLFFRYMAI GGYVGAATVG <br/>
AAAWWFLYAE DGPHVNYSQL THFMQCTEDN THFEGIDCEV FEAPEPMTMA <br/>
LSVLVTIEMC NALNSLSENQ SLLRMPPWVN IWLLGSICLS MSLHFLILYV <br/>
DPLPMIFKLR ALDLTQWLMV LKISLPVIGL DEILKFVARN YLEDPEDERR <br/>
K <br/> 
</font>

==Structural Information==
*Known structures with covalent ligands: <br/>
:Unknown<br/>

*Protein structure:
:Unknown<br/>

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04020 Calcium signaling patshway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04022 cGMP-PKG signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04024 cAMP signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04260 Cardiac muscle contraction] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04261 Adrenergic signaling in cardiomyocytes] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04919 Thyroid hormone signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04972 Pancreatic secretion] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05010 Alzheimer disease] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05410 Hypertrophic cardiomyopathy (HCM)] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05412 Arrhythmogenic right ventricular cardiomyopathy (ARVC)] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05414 Dilated cardiomyopathy (DCM)] <br/>

==Experimental Evidence==
:Cys-directed Mutation, Cys Modifiaction Assay

==Reference==
# Lytton J, Westlin M, Hanley M R. '''Thapsigargin inhibits the sarcoplasmic or endoplasmic reticulum Ca-ATPase family of calcium pumps[J].''' Journal of Biological Chemistry, 1991, 266(26): 17067-17071. [https://www.ncbi.nlm.nih.gov/pubmed/?term=1832668 1832668]<br/> 
# Duplan V, Serba C, Garcia J, et al. '''Synthesis of sesquiterpene-inspired derivatives designed for covalent binding and their inhibition of the NF-κB pathway[J].''' Organic & biomolecular chemistry, 2014, 12(2): 370-375. [https://www.ncbi.nlm.nih.gov/pubmed/?term=24263232 24263232]<br/> 
# Lancel S, Zhang J, Evangelista A, et al. '''Nitroxyl activates SERCA in cardiac myocytes via glutathiolation of cysteine 674[J].''' Circulation research, 2009, 104(6): 720-723. [https://www.ncbi.nlm.nih.gov/pubmed/?term=19265039 19265039]<br/> 
# Ying J, Tong X Y, Pimentel D R, et al. '''Cysteine-674 of the sarco/endoplasmic reticulum calcium ATPase is required for the inhibition of cell migration by nitric oxide[J].''' Arteriosclerosis, thrombosis, and vascular biology, 2007, 27(4): 783-790. [https://www.ncbi.nlm.nih.gov/pubmed/?term=17234728 17234728]<br/> 

[[Category:Targets]]
[[Category:Homo sapiens]]
[[Category:Ion channel]]
[[Category:Cation transport ATPase (P-type) family]]
[[Category:Type IIA subfamily]]
[[Category:Calcium signaling pathway]]
[[Category:cGMP-PKG signaling pathway]]
[[Category:cAMP signaling pathway]]
[[Category:Cardiac muscle contraction]]
[[Category:Adrenergic signaling in cardiomyocytes]]
[[Category:Thyroid hormone signaling pathway]]
[[Category:Pancreatic secretion]]
[[Category:Alzheimer disease]]
[[Category:Hypertrophic cardiomyopathy (HCM)]]
[[Category:Arrhythmogenic right ventricular cardiomyopathy (ARVC)]]
[[Category:Dilated cardiomyopathy (DCM)]]