View source for Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (Homo sapiens)

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{{#invoke:InfoboxforTarget|run|PP-1A, PPP1A, PPP1CA|[https://www.uniprot.org/uniprot/P62136 P62136]|Homo sapiens|Cys273|PPP phosphatase family,<br/>PP-1 subfamily|[[:Category:Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (Homo sapiens)|Ligand list]]|Phosphatase,<br/>Post-translational Modification}}

==Summary==

===Protein Function ===
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets.<br/>
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. (From Uniprot)
<br/>

===Cys Function & Property===
Cys273 is close to the active site and the manganese ions in space.<br/>

* Hydrophobic property:
:[[File:530-hydro.png||600px]]
* SASA：
:Cys273: 31.279 A^2

==Protein Sequence==
<font face="Courier">
MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP <br/>
ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS <br/>
LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK <br/>
TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL <br/>
LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV <br/>
VEDGYEFFAK RQLVTLFSAP NY<span style="background:#ffff00">'''C'''</span>GEFDNAG AMMSVDETLM CSFQILKPAD <br/>
KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK                       <br/> 
</font>

==Structural Information==
*Known structures with covalent ligands: <br/>
:Unknown<br/>

*Protein structure:
[[File:530.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko03015 mRNA surveillance pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04022 cGMP-PKG signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04024 cAMP signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04114 Oocyte meiosis] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04218 Cellular senescence] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04261 Adrenergic signaling in cardiomyocytes] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04270 Vascular smooth muscle contraction] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04390 Hippo signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04510 Focal adhesion] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04611 Platelet activation] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04720 Long-term potentiation] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04728 Dopaminergic synapse] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04750 Inflammatory mediator regulation of TRP channels] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04810 Regulation of actin cytoskeleton] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04910 Insulin signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04921 Oxytocin signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04931 Insulin resistance] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05031 Amphetamine addiction] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05034 Alcoholism] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05168 Herpes simplex virus 1 infection] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05205 Proteoglycans in cancer] <br/>


==Experimental Evidence==
:Cys-directed Mutation, Isotope Labeling   

==Reference==
# Pereira S R, Vasconcelos V M, Antunes A. '''Computational study of the covalent bonding of microcystins to cysteine residues–a reaction involved in the inhibition of the PPP family of protein phosphatases[J].''' The FEBS journal, 2013, 280(2): 674-680. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22177231 22177231]<br/> 
# MacKintosh R W, Dalby K N, Campbell D G, et al. '''The cyanobacterial toxin microcystin binds covalently to cysteine-273 on protein phosphatase 1[J].''' Febs Letters, 1995, 371(3): 236-240. [https://www.ncbi.nlm.nih.gov/pubmed/?term=7556599 7556599]<br/>
# Hastie C J, Borthwick E B, Morrison L F, et al. '''Inhibition of several protein phosphatases by a non-covalently interacting microcystin and a novel cyanobacterial peptide, nostocyclin[J].''' Biochimica et Biophysica Acta (BBA)-General Subjects, 2005, 1726(2): 187-193. [https://www.ncbi.nlm.nih.gov/pubmed/?term=16046071 16046071]<br/>

[[Category:Targets]]
[[Category:Homo sapiens]]
[[Category:Phosphatase]]
[[Category:Post-translational Modification]]
[[Category:PPP phosphatase family]]
[[Category:PP-1 subfamily]]
[[Category:mRNA surveillance pathway]]
[[Category:cGMP-PKG signaling pathway]]
[[Category:cAMP signaling pathway]]
[[Category:Oocyte meiosis]]
[[Category:Cellular senescence]]
[[Category:Adrenergic signaling in cardiomyocytes]]
[[Category:Vascular smooth muscle contraction]]
[[Category:Hippo signaling pathway]]
[[Category:Focal adhesion]]
[[Category:Platelet activation]]
[[Category:Long-term potentiation]]
[[Category:Dopaminergic synapse]]
[[Category:Inflammatory mediator regulation of TRP channels]]
[[Category:Regulation of actin cytoskeleton]]
[[Category:Insulin signaling pathway]]
[[Category:Oxytocin signaling pathway]]
[[Category:Insulin resistance]]
[[Category:Amphetamine addiction]]
[[Category:Alcoholism]]
[[Category:Herpes simplex virus 1 infection]]
[[Category:Proteoglycans in cancer]]