Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Basic Information
Short Name	SERCA2, SR Ca2+-ATPase 2, Calcium pump 2
UNP ID	P16615
Organism	Homo sapiens
Cys Site	Cys674
Family/Domain	Cation transport ATPase (P-type) family, Type IIA subfamily
Known Ligand	Ligand list
Function Type	Ion channel

Summary

Protein Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle. (From Uniprot)
SERCA Ca(2+)-ATPase is a intracellular pump located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. (From Wikipedia)

Cys Function & Property

Cys674 is very close to the ATP binding site in sequence.

• Hydrophobic property:

• SASA：

Cys674: Unknown

Protein Sequence

MENAHTKTVE EVLGHFGVNE STGLSLEQVK KLKERWGSNE LPAEEGKTLL
ELVIEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILVA
NAIVGVWQER NAENAIEALK EYEPEMGKVY RQDRKSVQRI KAKDIVPGDI
VEIAVGDKVP ADIRLTSIKS TTLRVDQSIL TGESVSVIKH TDPVPDPRAV
NQDKKNMLFS GTNIAAGKAM GVVVATGVNT EIGKIRDEMV ATEQERTPLQ
QKLDEFGEQL SKVISLICIA VWIINIGHFN DPVHGGSWIR GAIYYFKIAV
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
DKTGTLTTNQ MSVCRMFILD RVEGDTCSLN EFTITGSTYA PIGEVHKDDK
PVNCHQYDGL VELATICALC NDSALDYNEA KGVYEKVGEA TETALTCLVE
KMNVFDTELK GLSKIERANA CNSVIKQLMK KEFTLEFSRD RKSMSVYCTP
NKPSRTSMSK MFVKGAPEGV IDRCTHIRVG STKVPMTSGV KQKIMSVIRE
WGSGSDTLRC LALATHDNPL RREEMHLEDS ANFIKYETNL TFVGCVGMLD
PPRIEVASSV KLCRQAGIRV IMITGDNKGT AVAICRRIGI FGQDEDVTSK
AFTGREFDEL NPSAQRDACL NARCFARVEP SHKSKIVEFL QSFDEITAMT
GDGVNDAPAL KKAEIGIAMG SGTAVAKTAS EMVLADDNFS TIVAAVEEGR
AIYNNMKQFI RYLISSNVGE VVCIFLTAAL GFPEALIPVQ LLWVNLVTDG
LPATALGFNP PDLDIMNKPP RNPKEPLISG WLFFRYLAIG CYVGAATVGA
AAWWFIAADG GPRVSFYQLS HFLQCKEDNP DFEGVDCAIF ESPYPMTMAL
SVLVTIEMCN ALNSLSENQS LLRMPPWENI WLVGSICLSM SLHFLILYVE
PLPLIFQITP LNVTQWLMVL KISLPVILMD ETLKFVARNY LEPGKECVQP
ATKSCSFSAC TDGISWPFVL LIMPLVIWVY STDTNFSDMF WS

Structural Information

• Known structures with covalent ligands:
  

Unknown

• Protein structure:

Unknown

Related Pathway

• Calcium signaling patshway
  
• cGMP-PKG signaling pathway
  
• cAMP signaling pathway
  
• Cardiac muscle contraction
  
• Adrenergic signaling in cardiomyocytes
  
• Thyroid hormone signaling pathway
  
• Pancreatic secretion
  
• Alzheimer disease
  
• Hypertrophic cardiomyopathy (HCM)
  
• Arrhythmogenic right ventricular cardiomyopathy (ARVC)
  
• Dilated cardiomyopathy (DCM)
  

Experimental Evidence

Cys-directed Mutation, Cys Modifiaction Assay

Reference

1. Lancel S, Zhang J, Evangelista A, et al. Nitroxyl activates SERCA in cardiac myocytes via glutathiolation of cysteine 674[J]. Circulation research, 2009, 104(6): 720-723. 19265039