Legumain

Basic Information
Short Name	LGMN, PRSC1
UNP ID	Q99538
Organism	Homo sapiens
Cys Site	Cys189
Family/Domain	Peptidase C13 family
Known Ligand	Ligand list
Function Type	Protease

Summary

Protein Function

Legumain is a cysteine protease from the C13 family of the CD clan of proteases (MEROPS). It uses a catalytic triad of Cysteine-Histidine-Asparagine in its active site to perform covalent proteolysis of its substrate. It catalyses the following chemical reaction: Hydrolysis of proteins and small molecule substrates at -Asn-Xaa- bonds. Both plant and animal legumains are most active in acidic environments. (From Wikipedia)

Cys Function & Property

Cys189 is one of the active sites of Legumain.

• Hydrophobic property:

• SASA：

Cys189: 4.399 A^2

Protein Sequence

MVWKVAVFLS VALGIGAVPI DDPEDGGKHW VVIVAGSNGW YNYRHQADAC
HAYQIIHRNG IPDEQIVVMM YDDIAYSEDN PTPGIVINRP NGTDVYQGVP
KDYTGEDVTP QNFLAVLRGD AEAVKGIGSG KVLKSGPQDH VFIYFTDHGS
TGILVFPNED LHVKDLNETI HYMYKHKMYR KMVFYIEACE SGSMMNHLPD
NINVYATTAA NPRESSYACY YDEKRSTYLG DWYSVNWMED SDVEDLTKET
LHKQYHLVKS HTNTSHVMQY GNKTISTMKV MQFQGMKRKA SSPVPLPPVT
HLDLTPSPDV PLTIMKRKLM NTNDLEESRQ LTEEIQRHLD ARHLIEKSVR
KIVSLLAASE AEVEQLLSER APLTGHSCYP EALLHFRTHC FNWHSPTYEY
ALRHLYVLVN LCEKPYPLHR IKLSMDHVCL GHY

Structural Information

• Known structure with covalent ligand:
  

4AWB, 4AW9, 4AWA

• Protein structure:

Related Pathway

• Lysosome
  
• Antigen processing and presentation
  

Experimental Evidence

Crystallography

Reference

1. Lee J, Bogyo M. Synthesis and evaluation of aza-peptidyl inhibitors of the lysosomal asparaginyl endopeptidase, legumain[J]. Bioorganic & medicinal chemistry letters, 2012, 22(3): 1340-1343. 22243962
   
2. Dall E, Brandstetter H. Mechanistic and structural studies on legumain explain its zymogenicity, distinct activation pathways, and regulation[J]. Proceedings of the National Academy of Sciences, 2013, 110(27): 10940-10945. 23776206