Ubiquitin-conjugating enzyme E2 D2

Basic Information
Short Name	UBE2D2
UNP ID	P62837
Organism	Homo sapiens
Cys Site	Cys85
Family/Domain	Ubiquitin-conjugating enzyme family
Known Ligand	Ligand list
Function Type	Post-translational Modification, Ubiquitinase/Deubiquitinase

Summary

Protein Function

Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell.
UBE2D2 expressed in 236 organs, highest expression level in testis.

PMID: 27002218

Cys Function & Property

Cys85 is the active site of UBE2D2, act as a glycyl thioester intermediate.

• Hydrophobic property:

• SASA:

Cys85: 18.529 A^2

Protein Sequence

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM

Structural Information

• Known structure with covalent ligand:
  

Unknown

• Protein structure:

Related Pathway

• Ubiquitin mediated proteolysis
  
• Protein processing in endoplasmic reticulum
  
• Shigellosis
  

Experimental Evidence

LC-CID-MS/MS, Tryptic Digest

Reference

1. Liu L, Hua Y, Wang D, et al. A sesquiterpene lactone from a medicinal herb inhibits proinflammatory activity of TNF-α by inhibiting ubiquitin-conjugating enzyme UbcH5[J]. Chemistry & biology, 2014, 21(10): 1341-1350. 25200604