Hsp90 co-chaperone Cdc37

Basic Information
Short Name	CDC37
UNP ID	Q16543
Organism	Homo sapiens
Cys Site	Cys57, Cys54, Cys64
Family/Domain	CDC37 family
Known Ligand	Ligand list
Function Type	Chaperone

Summary

Protein Function

Hsp90 co-chaperone Cdc37 is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK, as well as eIF-2 alpha kinases, and is upregulated in various cancers. It is thought to play a critical role in directing Hsp90 to its target kinases. The protein–protein complex forms with a KD value of 1.2 mm and is considered to mediate carcinogenesis by stabilizing a variety of different oncogenic kinases in malignant cells.
CDC37 consists of three structural domains. The N-terminal domain binds to protein kinases. The central domain is the Hsp90chaperone (heat shock protein 90) binding domain. The function of the C-terminal domain is unclear. (From Uniprot, Wikipedia, PMID: 19585625)

Cys Function & Property

From the mutation, homology modeling and other assay, Cys57, Cys54, Cys64 are confirmed to located in/near the celastrol binding pocket.

Possible mechanisms of inactivation of Cdc37 by celastrol

The predicted model of Cdc37_NM complexed with celastrol

• Hydrophobic property:

• SASA:

Cys57, Cys54, Cys64

10.925 A^2

Protein Sequence

MVDYSVWDHI EVSDDEDETH PNIDTASLFR WRHQARVERM EQFQKEKEEL
DRGCRECKRK VAECQRKLKE LEVAEGGKAE LERLQAEAQQ LRKEERSWEQ
KLEEMRKKEK SMPWNVDTLS KDGFSKSMVN TKPEKTEEDS EEVREQKHKT
FVEKYEKQIK HFGMLRRWDD SQKYLSDNVH LVCEETANYL VIWCIDLEVE
EKCALMEQVA HQTIVMQFIL ELAKSLKVDP RACFRQFFTK IKTADRQYME
GFNDELEAFK ERVRGRAKLR IEKAMKEYEE EERKKRLGPG GLDPVEVYES
LPEELQKCFD VKDVQMLQDA ISKMDPTDAK YHMQRCIDSG LWVPNSKASE
AKEGEEAGPG DPLLEAVPKT GDEKDVSV

Structural Information

• Known structure with covalent ligand:
  

Unknown

• Protein structure:

Unknown

Related Pathway

• PI3K-Akt signaling pathway
  

Experimental Evidence

Nuclear Magnetic Resonance, Mass Spectrometry

Reference

1. Sreeramulu S, Gande S L, Göbel M, et al. Molecular mechanism of inhibition of the human protein complex Hsp90–Cdc37, a kinome chaperone–cochaperone, by triterpene celastrol[J]. Angewandte Chemie International Edition, 2009, 48(32): 5853-5855. 19585625
   
2. Duan Y, Jin H, Yu H, et al. Computational investigation of interactions between Cdc37 and celastrol[J]. Molecular Simulation, 2013, 39(4): 270-278. DOI:10.1080/08927022.2012.718439.