Caspase-7

Basic Information
Short Name	CASP7
UNP ID	P55210
Organism	Homo sapiens
Cys Site	Cys186
Family/Domain	Peptidase C14A family
Known Ligand	Ligand list
Function Type	Protease

Summary

Protein Function

Caspases (cysteine-aspartic acid protease (Caspase) family) are a family of cysteine proteases that have important intracellular roles in inflammation and apoptosis. Activation of Caspases ensures that the cellular components are degraded in a controlled manner, carrying out cell death with minimal effect on surrounding tissues. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of Caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits.
Caspase-7 is a member of the Caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Sequential activation of Caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing by upstream Caspases (Caspase-8, -9) at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme in the form of a heterotetramer. The precursor of this Caspase is cleaved by Caspase 3, Caspase 10, and Caspase 9. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. (From Wikipedia)
Involved in the activation cascade of Caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death. (From Uniprot)

Cys Function & Property

The catalytic triad in Caspase-7 comprises Cys186, His144 and the backbone carbonyl oxygen atom of Arg87, which points towards the Nϵ atom of His144.

• Hydrophobic property:

• SASA:

Cys186: 14.133 A^2

Protein Sequence

MADDQGCIEE QGVEDSANED SVDAKPDRSS FVPSLFSKKK KNVTMRSIKT
TRDRVPTYQY NMNFEKLGKC IIINNKNFDK VTGMGVRNGT DKDAEALFKC
FRSLGFDVIV YNDCSCAKMQ DLLKKASEED HTNAACFACI LLSHGEENVI
YGKDGVTPIK DLTAHFRGDR CKTLLEKPKL FFIQACRGTE LDDGIQADSG
PINDTDANPR YKIPVEADFL FAYSTVPGYY SWRSPGRGSW FVQALCSILE
EHGKDLEIMQ ILTRVNDRVA RHFESQSDDP HFHEKKQIPC VVSMLTKELY
FSQ

Structural Information

• Known structure with covalent ligand:
  

1F1J

3IBC

• Protein structure:

Related Pathway

• Apoptosis
  
• Apoptosis - multiple species
  
• TNF signaling pathway
  
• Non-alcoholic fatty liver disease (NAFLD)
  
• Alzheimer disease
  
• Pathogenic Escherichia coli infection
  
• Pertussis
  
• Legionellosis
  
• Pathways in cancer
  

Experimental Evidence

Crystallography

Reference

1. Agniswamy J, Fang B, Weber I T. Conformational similarity in the activation of caspase-3 and-7 revealed by the unliganded and inhibited structures of caspase-7[J]. Apoptosis, 2009, 14(10): 1135-1144. 19655253
   
2. Y Wei, T Fox, S P Chambers et al. The structures of caspases-1, -3, -7 and -8 reveal the basis for substrate and inhibitor selectivity. Chem Biol, 2000, 7(6): 423-432. 10873833