Ubiquitin-conjugating enzyme E2 D3

Basic Information
Short Name	UBE2D3
UNP ID	P61077
Organism	Homo sapiens
Cys Site	Cys85
Family/Domain	Ubiquitin-conjugating enzyme family
Known Ligand	Ligand list
Function Type	Post-translational Modification, Ubiquitinase/Deubiquitinase

Summary

Protein Function

Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell.

PMID: 27002218

Cys Function & Property

Cys85 is the active site of UBE2D3, act as a glycyl thioester intermediate.

• Hydrophobic property:

• SASA:

Cys85: 22.494 A^2

Protein Sequence

MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
KVLLSICSLL CDPNPDDPLV PEIARIYKTD RDKYNRISRE WTQKYAM

Structural Information

• Known structure with covalent ligand:
  

Unknown

• Protein structure:

Related Pathway

• Ubiquitin mediated proteolysis
  
• Protein processing in endoplasmic reticulum
  
• Shigellosis
  

Experimental Evidence

LC-CID-MS/MS, Tryptic Digest

Reference

1. Liu L, Hua Y, Wang D, et al. A sesquiterpene lactone from a medicinal herb inhibits proinflammatory activity of TNF-α by inhibiting ubiquitin-conjugating enzyme UbcH5[J]. Chemistry & biology, 2014, 21(10): 1341-1350. 25200604