Cathepsin S

Summary

Protein Function

Cathepsin S is a thiol protease, which act as key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound. (From Uniprot)

Cys Function & Property

Cys139 is one of the active sites of Cathepsin S, which is very close to His278 and Asn298 in space. These three residues formed a typical catalytic triad motif.

• Hydrophobic property:

• SASA：

Cys139: 11.884 A^2

Protein Sequence

MKRLVCVLLV CSSAVAQLHK DPTLDHHWHL WKKTYGKQYK EKNEEAVRRL
IWEKNLKFVM LHNLEHSMGM HSYDLGMNHL GDMTSEEVMS LMSSLRVPSQ
WQRNITYKSN PNRILPDSVD WREKGCVTEV KYQGSCGACW AFSAVGALEA
QLKLKTGKLV SLSAQNLVDC STEKYGNKGC NGGFMTTAFQ YIIDNKGIDS
DASYPYKAMD QKCQYDSKYR AATCSKYTEL PYGREDVLKE AVANKGPVSV
GVDARHPSFF LYRSGVYYEP SCTQNVNHGV LVVGYGDLNG KEYWLVKNSW
GHNFGEEGYI RMARNKGNHC GIASFPSYPE I

Structural Information

• Known structures with covalent ligands:
  

1NPZ, 3N4C, 2OP3

• Protein structure:

Related Pathway

• Lysosome
  
• Phagosome
  
• Tuberculosis
  
• Antigen processing and presentation
  

Experimental Evidence

Crystallography, Cys-directed mutation, Homologous Analysis of Sequence, Enzymatic Assay

Reference

1. Pauly T A, Sulea T, Ammirati M, et al. Specificity determinants of human cathepsin S revealed by crystal structures of complexes[J]. Biochemistry, 2003, 42(11): 3203-3213. 12641451
   
2. Cai J, Baugh M, Black D, et al. 6-Phenyl-1H-imidazo [4, 5-c] pyridine-4-carbonitrile as cathepsin S inhibitors[J]. Bioorganic & Medicinal Chemistry Letters, 2010, 20(15): 4350-4354. 20598883
   
3. Inagaki H, Tsuruoka H, Hornsby M, et al. Characterization and optimization of selective, nonpeptidic inhibitors of cathepsin S with an unprecedented binding mode[J]. Journal of Medicinal Chemistry, 2007, 50(11): 2693-2699. 17469812
   
4. Bekkali Y, Thomson D S, Betageri R, et al. Identification of a novel class of succinyl-nitrile-based Cathepsin S inhibitors[J]. Bioorganic & Medicinal Chemistry Letters, 2007, 17(9): 2465-2469. 17379516