Mitochondrial carnitine/acylcarnitine carrier protein
| Basic Information | |
|---|---|
| Short Name | CAC, Slc25a20 |
| UNP ID | P97521 |
| Organism | Rattus norvegicus |
| Cys Site | Cys136, Cys283 |
| Family/Domain | Mitochondrial carrier family |
| Known Ligand | Ligand list |
| Function Type | Transporter |
Summary
Protein Function
This enzyme is required since fatty acids cannot cross the mitochondrial membranes without assistance. The fatty acid is firstly bound to CoA and may cross the external mitochondrial membrane. It then exchanges the CoA for carnitine by the action of the enzyme carnitine palmitoyltransferase I. The complex then enters the mitochondrial matrix thanks to facilitated diffusion by carnitine-acylcarnitine translocase. Here, the acyl-cartinine complex is disrupted by carnitine palmitoyltransferase II and the fatty acid rebinds to CoA. Carnitine then diffuses back across the membrane by carnitine-acylcarnitine translocase into the mitochondrial intermembrane space. This is called the carnitine shuttle system. (From Wiki)
Mediates the transport of acylcarnitines of different length across the mitochondrial inner membrane from the cytosol to the mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway. (From Uniprot)
CAC is functionally and structurally asymmetrical and it is inserted in the proteoliposomal membrane in a right-side out orientation as compared to mitochondria. (PMID: 24349247)
Cys Function & Property
To be added
- Hydrophobic property:
- SASA：
- Cys136: 12.115 A^2
- Cys283: 20.751 A^2
Protein Sequence
MAEEPKPISP LKNLLAGGFG GVCLVFVGHP LDTVKVRLQT QPPSLPGQPP
MYSGTIDCFR KTLFREGITG LYRGMAAPII GVTPMFAVCF FGFGLGKRLQ
QKSPEDELTY PQLFTAGMLS GVFTTGIMTP GERIKCLLQI QASSGKNKYS
GTLDCAKKLY QEFGIRGFYK GTALTLMRDV PASGMYFMTY EWLKNLFTPQ
GKSVHDLSVP RVLVAGGFRG IFNWVVAIPP DVLKSRFQTA PPGKYPNGFR
DVLRELIREE GVTSLYKGFN AVMIRAFPAN AACFLGFEIP MKILNWIAPN
L
Structural Information
- Known structures with covalent ligands:
- Unknown
- Protein structure:
Related Pathway
- Unknown
Experimental Evidence
- Cys-directed mutation, Molecular Docking
Reference
- Tonazzi A, Eberini I, Indiveri C. Molecular mechanism of inhibition of the mitochondrial carnitine/acylcarnitine transporter by omeprazole revealed by proteoliposome assay, mutagenesis and bioinformatics[J]. PloS one, 2013, 8(12): e82286. 24349247
