Sodium/bile acid cotransporter

Basic Information
Short Name	SLC10A1
UNP ID	Q14973
Organism	Homo sapiens
Cys Site	Cys96, Cys266
Family/Domain	Bile acid:sodium symporter family
Known Ligand	Ligand list
Function Type	Transporter

Summary

Protein Function

The Na⁺-dependent taurocholate (TC) cotransporting polypeptide (NTCP/Ntcp) is the major sinusoidal bile salt transporter of hepatocytes. Ntcp mediates the transport of conjugated bile salts such as TC and taurochenodeoxycholate in a Na⁺-dependent fashion using the Na⁺ gradient produced by the Na⁺-K⁺-ATPase. (PMID: 23886862)

Distribution of cysteine residues

Cys Function & Property

Ntcp has 8 cysteines, and one of these, Cys266, is highly conserved not only across several species but also with the NTCP/Ntcp homolog, apical Na⁺-dependent bile acid transporter (Asbt).
Some three-dimensional homology model for human NTCP predicts that Cys96 lies in a possible permeation pathway for Na⁺. And S-nitrosylation of Cys96 may result in steric hindrance that blocks the permeation pathway for Na⁺ and thereby inhibits Ntcp function.

• Hydrophobic property:

• SASA：

Unknown

Protein Sequence

MEAHNASAPF NFTLPPNFGK RPTDLALSVI LVFMLFFIML SLGCTMEFSK
IKAHLWKPKG LAIALVAQYG IMPLTAFVLG KVFRLKNIEA LAILVCGCSP
GGNLSNVFSL AMKGDMNLSI VMTTCSTFCA LGMMPLLLYI YSRGIYDGDL
KDKVPYKGIV ISLVLVLIPC TIGIVLKSKR PQYMRYVIKG GMIIILLCSV
AVTVLSAINV GKSIMFAMTP LLIATSSLMP FIGFLLGYVL SALFCLNGRC
RRTVSMETGC QNVQLCSTIL NVAFPPEVIG PLFFFPLLYM IFQLGEGLLL
IAIFWCYEKF KTPKDKTKMI YTAATTEETI PGALGNGTYK GEDCSPCTA

Structural Information

• Known structure with covalent ligand:
  

Unknown

• Protein structure:

Unknown

Related Pathway

• Bile secretion
  
• Hepatitis B
  

Experimental Evidence

Cys-directed Mutation, Homology Modeling

Reference

1. Ramasamy U, Anwer M S, Schonhoff C M. Cysteine 96 of Ntcp is responsible for NO-mediated inhibition of taurocholate uptake[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2013, 305(7): G513-G519. 23886862