Tubulin alpha-1A chain (Sus scrofa)
| Basic Information | |
|---|---|
| Short Name | TUBA1A, Alpha-tubulin 1 |
| UNP ID | P02550 |
| Organism | Sus scrofa |
| Cys Site | Cys213 |
| Family/Domain | Tubulin family |
| Known Ligand | Ligand list |
| Function Type | Structural protein |
Summary
Protein Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. (From Uniprot)
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of α and β tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the α, β and γ tubulin families are found in all eukaryotes. The α and β tubulins represent the major components of microtubules, while γ tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple α and β tubulin genes, which are highly conserved among species. (From Wikipedia)
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. (From Uniprot)
Cys Function & Property
Cys213 is close to the GTP binding site and the dimer interface.
- Hydrophobic property:
- SASA：
- Cys213: 0.818 A^2
Protein Sequence
MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN
TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA
NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFSVFHS FGGGTGSGFT
SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC
AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA SLRFDGALNV
DLTEFQTNLV PYPRAHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN
QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG
FKVGINYEPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA
KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE
Y
Structural Information
- Known structure with covalent ligand:
- Unknown
- Protein structure:
Related Pathway
Experimental Evidence
- DTNB Method, Immunocytochemical Staining
Reference
- Ishiguro K, Ando T, Watanabe O, et al. Specific reaction of α, β-unsaturated carbonyl compounds such as 6-shogaol with sulfhydryl groups in tubulin leading to microtubule damage[J]. FEBS letters, 2008, 582(23-24): 3531-3536. 18805415
