View source for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

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{{#invoke:InfoboxforTarget|run|PP2A-alpha, PPP2CA,<br/>RP-C|[https://www.uniprot.org/uniprot/P67775 P67775]|Homo sapiens|Cys269|PPP phosphatase family,<br/>PP-1 subfamily|[[:Category:Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform|Ligand list]]|Phosphatase,<br/>Post-translational Modification}}

==Summary==

===Protein Function ===
PP2A consists of a dimeric core enzyme composed of the structural A and catalytic C subunits, and a regulatory B subunit. When the PP2A catalytic C subunit associates with the A and B subunits several species of holoenzymes are produced with distinct functions and characteristics. The A subunit, a founding member of the HEAT repeat protein family (huntington-elongation-A subunit-TOR), is the scaffold required for the formation of the heterotrimeric complex. When the A subunit binds it alters the enzymatic activity of the catalytic subunit, even if the B subunit is absent. While C and A subunit sequences show remarkable sequence conservation throughout eukaryotes, regulatory B subunits are more heterogeneous and are believed to play key roles in controlling the localization and specific activity of different holoenzymes. (From Wikipedia)
<br/>

===Cys Function & Property===
Cys273 is close to the active site and the manganese ions in space.<br/>

* Hydrophobic property:
:[[File:531-hydro.png||600px]]
* SASA：
:Cys273: 31.279 A^2

==Protein Sequence==
<font face="Courier">
MDEKVFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC <br/>
PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL <br/>
VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF <br/>
DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW <br/>
SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW <br/>
CHDRNVVTIF SAPNYCYR<span style="background:#ffff00">'''C'''</span>G NQAAIMELDD TLKYSFLQFD PAPRRGEPHV <br/>
TRRTPDYFL             <br/> 
</font>

==Structural Information==
*Known structures with covalent ligands: <br/>
:[https://www.rcsb.org/structure/2IE3 2IE3], [https://www.rcsb.org/structure/4I5N 4I5N]<br/>

*Protein structure:
[[File:531.png|center|800px]]

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko03015 mRNA surveillance pathway] <br/>

*[https://www.genome.jp/kegg-bin/show_pathway?ko03015 mRNA surveillance pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04071 Sphingolipid signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04114 Oocyte meiosis] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04140 Autophagy - animal] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04151 PI3K-Akt signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04152 AMPK signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04261 Adrenergic signaling in cardiomyocytes] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04350 TGF-beta signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04390 Hippo signaling pathway] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04530 Tight junction] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04728 Dopaminergic synapse] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04730 Long-term depression] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05142 Chagas disease (American trypanosomiasis)] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05160 Hepatitis C] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko05165 Human papillomavirus infection]

==Experimental Evidence==
:Cys-directed Mutation, Isotope Labeling   

==Reference==
# Pereira S R, Vasconcelos V M, Antunes A. '''Computational study of the covalent bonding of microcystins to cysteine residues–a reaction involved in the inhibition of the PPP family of protein phosphatases[J].''' The FEBS journal, 2013, 280(2): 674-680. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22177231 22177231]<br/> 
# MacKintosh R W, Dalby K N, Campbell D G, et al. '''The cyanobacterial toxin microcystin binds covalently to cysteine-273 on protein phosphatase 1[J].''' Febs Letters, 1995, 371(3): 236-240. [https://www.ncbi.nlm.nih.gov/pubmed/?term=7556599 7556599]<br/>
# Hastie C J, Borthwick E B, Morrison L F, et al. '''Inhibition of several protein phosphatases by a non-covalently interacting microcystin and a novel cyanobacterial peptide, nostocyclin[J].''' Biochimica et Biophysica Acta (BBA)-General Subjects, 2005, 1726(2): 187-193. [https://www.ncbi.nlm.nih.gov/pubmed/?term=16046071 16046071]<br/>

[[Category:Targets]]
[[Category:Homo sapiens]]
[[Category:Phosphatase]]
[[Category:Post-translational Modification]]
[[Category:PPP phosphatase family]]
[[Category:PP-1 subfamily]]
[[Category:mRNA surveillance pathway]]
[[Category:Sphingolipid signaling pathway]]
[[Category:Oocyte meiosis]]
[[Category:Autophagy - animal]]
[[Category:PI3K-Akt signaling pathway]]
[[Category:AMPK signaling pathway]]
[[Category:Adrenergic signaling in cardiomyocytes]]
[[Category:TGF-beta signaling pathway]]
[[Category:Hippo signaling pathway]]
[[Category:Tight junction]]
[[Category:Dopaminergic synapse]]
[[Category:Long-term depression]]
[[Category:Chagas disease]]
[[Category:Hepatitis C]]
[[Category:Human papillomavirus infection]]