View source for Sodium- and chloride-dependent GABA transporter 1

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{{#invoke:InfoboxforTarget|run|SLC6A1, GABATR,<br/>GABT1, GAT1|[https://www.uniprot.org/uniprot/P30531 P30531]|Homo sapiens|Cys74|[http://pfam.xfam.org/family/PF00209 Sodium:neurotransmitter symporter (SNF) family], SLC6A1 subfamily|[[:Category:Sodium- and chloride-dependent GABA transporter 1|Ligand list]]|Transporter}}
==Summary==

===Protein Function ===
In the nervous system, removal of the inhibitory neurotransmitter c-aminobutyric acid (GABA) from the extracellular space of neurons and glia is accomplished by electrogenic Na<sup>+</sup>- and Cl<sup>-</sup>-coupled GABA transporters (GATs). GAT1 was the first family member to be cloned by Guastella and Nelson in 1990. And it is the most abundant isoform within the nervous system and is expressed in both neurons and glia. <br/>
Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
<br/>

===Cys Function & Property===
Cys74, which located in the hydrophilic loop connecting transmembrane domains 1 and 2, is one of the extracellularly exposed, endogenous cysteine residue of GAT1, has been used to gain insight into the nature and functional consequences of sulfhydryl modification at this residue in many works, such as Bennett and Kanner 1997 (PMID: [https://www.ncbi.nlm.nih.gov/pubmed/?term=8995422 8995422]), Yu et al. 1998 (PMID:[https://www.ncbi.nlm.nih.gov/pubmed/?term=9599002 9599002]), Golovanevsky and Kanner 1999 (PMID: [https://www.ncbi.nlm.nih.gov/pubmed/?term=10438469 10438469]), Li et al. 2000 (PMID: [https://www.ncbi.nlm.nih.gov/pubmed/?term=10736315 10736315]). 
[[File:590-function.png|center|600px]]
<div align="center">Topology and cysteine residues of GAT1</div><br/>

* Hydrophobic property:
:[[File:590-hydro.png||600px]]
* SASA:
:Unknown<br/>

==Protein Sequence==
<font face="Courier">
MATNGSKVAD GQISTEVSEA PVANDKPKTL VVKVQKKAAD LPDRDTWKGR <br/>
FDFLMSCVGY AIGLGNVWRF PYL<span style="background:#ffff00">'''C'''</span>GKNGGG AFLIPYFLTL IFAGVPLFLL <br/>
ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY <br/>
LYNSFTTTLP WKQCDNPWNT DRCFSNYSMV NTTNMTSAVV EFWERNMHQM <br/>
TDGLDKPGQI RWPLAITLAI AWILVYFCIW KGVGWTGKVV YFSATYPYIM <br/>
LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL <br/>
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK <br/>
RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT <br/>
VEGFITALVD EYPRLLRNRR ELFIAAVCII SYLIGLSNIT QGGIYVFKLF <br/>
DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW <br/>
SFFTPIIVAG VFIFSAVQMT PLTMGNYVFP KWGQGVGWLM ALSSMVLIPG <br/>
YMAYMFLTLK GSLKQRIQVM VQPSEDIVRP ENGPEQPQAG SSTSKEAYI  <br/> 
</font>

==Structural Information==
*Known structure with covalent ligand: <br/>
:Unknown<br/>

*Protein structure:
:Unknown<br/>

==Related Pathway==
*[https://www.genome.jp/kegg-bin/show_pathway?ko04721 Synaptic vesicle cycle] <br/>
*[https://www.genome.jp/kegg-bin/show_pathway?ko04727 GABAergic synapse] <br/>

==Experimental Evidence==
:Cys-directed mutation, Homologous Analysis Of Sequence, Molecular Docking

==Reference==
# Omoto J J, Maestas M J, Rahnama-Vaghef A, et al. '''Functional consequences of sulfhydryl modification of the γ-aminobutyric acid transporter 1 at a single solvent-exposed cysteine residue[J].''' The Journal of membrane biology, 2012, 245(12): 841-857. [https://www.ncbi.nlm.nih.gov/pubmed/?term=22918627 22918627]<br/> 

[[Category:Targets]]
[[Category:Homo sapiens]]
[[Category:Transporter]]
[[Category:Sodium:neurotransmitter symporter (SNF) family]]
[[Category:SLC6A1 subfamily]]
[[Category:Synaptic vesicle cycle]]
[[Category:GABAergic synapse]]